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IRTG Lecture - Dr. Mirko Vanetti

EU Application Manager – Functional Genomics IDT Integrated DNA Technologies


Title: Mitigating the risk of off-target effects when using CRISPR genome editing

• Part I –Introduction to the plasmid-free CRISPR/Cas9 RNP (ribonucleoprotein) workflow.

From the guide design to the analysis of editing events.

  • Knocking out genes
  • Knocking in new sequence information

• Part II – Increasing specificity of CRISPR

  • Identifying and reducing off-target effects in CRISPR
  • recent developments in CRISPR research, including a novel mutant Cas9 protein that has been evolved to reduce off-target gene editing while maintaing on-target potency


Room: Room N 02.017
BioMedical Center, Großhaderner Str. 9, Planegg-Martinsried


This lecture is part of the IRTG Lecture Series "State-of-the-art Research Methods in Immunology" and is open to all interested students, doctoral and postdoctoral researchers.