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Voboril, M., Brabec, T., Dobes, J., Splichalova, I., Brezina, J., Cepkova, A., Dobesova, M., Aidarova, A., Kubovciak, J., Tsyklauri, O., Stepanek, O., Benes, V., Sedlacek, R., Klein, L., Kolar, M., and Filipp, D. (2020). Toll-like receptor signaling in thymic epithelium controls monocyte-derived dendritic cell recruitment and Treg generation. Nat Commun 11:2361.
PMID: 32398640,

Schober, K., Voit, F., Grassmann, S., Muller, T.R., Eggert, J., Jarosch, S., Weissbrich, B., Hoffmann, P., Borkner, L., Nio, E., Fanchi, L., Clouser, C.R., Radhakrishnan, A., Mihatsch, L., Luckemeier, P., Leube, J., Dossinger, G., Klein, L., Neuenhahn, M., Oduro, J.D., Cicin-Sain, L., Buchholz, V.R., and Busch, D.H. (2020). Reverse TCR repertoire evolution toward dominant low-affinity clones during chronic CMV infection. Nat Immunol 21:434-441.
PMID: 32205883,


Yamano, T., Steinert, M., Steer, B., Klein, L., Hammerschmidt, W., and Adler, H. (2019a). B cells latently infected with murine gammaherpesvirus 68 (MHV-68) are present in the mouse thymus-A step toward immune evasion? Eur J Immunol 49:351-352.
PMID: 30488950,

Yamano, T., Dobes, J., Voboril, M., Steinert, M., Brabec, T., Zietara, N., Dobesova, M., Ohnmacht, C., Laan, M., Peterson, P., Benes, V., Sedlacek, R., Hanayama, R., Kolar, M., Klein, L., and Filipp, D. (2019b). Aire-expressing ILC3-like cells in the lymph node display potent APC features. J Exp Med 216:1027-1037.
PMID: 30918005,

Klein, L., Robey, E.A., and Hsieh, C.S. (2019). Central CD4(+) T cell tolerance: deletion versus regulatory T cell differentiation. Nat Rev Immunol 19:7-18.
PMID: 30420705,

Hassler, T., Urmann, E., Teschner, S., Federle, C., Dileepan, T., Schober, K., Jenkins, M.K., Busch, D.H., Hinterberger, M., and Klein, L. (2019). Inventories of naive and tolerant mouse CD4 T cell repertoires reveal a hierarchy of deleted and diverted T cell receptors. Proc Natl Acad Sci U S A 116:18537-18543.
PMID: 31451631,


Dobes, J., Edenhofer, F., Voboril, M., Brabec, T., Dobesova, M., Cepkova, A., Klein, L., Rajewsky, K., and Filipp, D. (2018). A novel conditional Aire allele enables cell-specific ablation of the immune tolerance regulator Aire. Eur J Immunol 48:546-548.
PMID: 29193031,


Wang, L., Winnewisser, J., Federle, C., Jessberger, G., Nave, K.A., Werner, H.B., Kyewski, B., Klein, L., and Hinterberger, M. (2017). Epitope-Specific Tolerance Modes Differentially Specify Susceptibility to Proteolipid Protein-Induced Experimental Autoimmune Encephalomyelitis. Front Immunol 8:1511.
PMID: 29170668,


von Rohrscheidt, J., Petrozziello, E., Nedjic, J., Federle, C., Krzyzak, L., Ploegh, H.L., Ishido, S., Steinkasserer, A., and Klein, L. (2016). Thymic CD4 T cell selection requires attenuation of March8-mediated MHCII turnover in cortical epithelial cells through CD83. J Exp Med 213:1685-1694.
PMID: 27503071,

Pasquarella, A., Ebert, A., Pereira de Almeida, G., Hinterberger, M., Kazerani, M., Nuber, A., Ellwart, J., Klein, L., Busslinger, M., and Schotta, G. (2016). Retrotransposon derepression leads to activation of the unfolded protein response and apoptosis in pro-B cells. Development 143:1788-1799.
PMID: 27013243,


Yamano, T., Steinert, M., and Klein, L. (2015a). Thymic B Cells and Central T Cell Tolerance. Front Immunol 6:376.
PMID: 26257742,

Yamano, T., Nedjic, J., Hinterberger, M., Steinert, M., Koser, S., Pinto, S., Gerdes, N., Lutgens, E., Ishimaru, N., Busslinger, M., Brors, B., Kyewski, B., and Klein, L. (2015b). Thymic B Cells Are Licensed to Present Self Antigens for Central T Cell Tolerance Induction. Immunity 42:1048-1061.
PMID: 26070482,

Warth, S.C., Hoefig, K.P., Hiekel, A., Schallenberg, S., Jovanovic, K., Klein, L., Kretschmer, K., Ansel, K.M., and Heissmeyer, V. (2015). Induced miR-99a expression represses Mtor cooperatively with miR-150 to promote regulatory T-cell differentiation. EMBO J 34:1195-1213.
PMID: 25712478,

Klein, L. (2015). Aire Gets Company for Immune Tolerance. Cell 163:794-795.
PMID: 26544932,


Vahl, J.C., Drees, C., Heger, K., Heink, S., Fischer, J.C., Nedjic, J., Ohkura, N., Morikawa, H., Poeck, H., Schallenberg, S., Riess, D., Hein, M.Y., Buch, T., Polic, B., Schonle, A., Zeiser, R., Schmitt-Graff, A., Kretschmer, K., Klein, L., Korn, T., Sakaguchi, S., and Schmidt-Supprian, M. (2014). Continuous T cell receptor signals maintain a functional regulatory T cell pool. Immunity 41:722-736.
PMID: 25464853,

Klein, L., Kyewski, B., Allen, P.M., and Hogquist, K.A. (2014). Positive and negative selection of the T cell repertoire: what thymocytes see (and don't see). Nat Rev Immunol 14:377-391.
PMID: 24830344,


Wu, C., Aichinger, M., Nedjic, J., and Klein, L. (2013). Thymic epithelial cells use macroautophagy to turn their inside out for CD4 T cell tolerance. Autophagy 9:931-932.
PMID: 23548947,

Aichinger, M., Wu, C., Nedjic, J., and Klein, L. (2013). Macroautophagy substrates are loaded onto MHC class II of medullary thymic epithelial cells for central tolerance. J Exp Med 210:287-300.
PMID: 23382543,


Klein, L., and Jovanovic, K. (2012). Regulatory T cell differentiation: turning harmful into useful. Immunity 37:441-443.
PMID: 22999949,

Aichinger, M., Hinterberger, M., and Klein, L. (2012). Probing gene function in thymic epithelial cells. Eur J Cell Biol 91:24-30.
PMID: 21392839,


Wirnsberger, G., Hinterberger, M., and Klein, L. (2011). Regulatory T-cell differentiation versus clonal deletion of autoreactive thymocytes. Immunol Cell Biol 89:45-53.
PMID: 21042335,

Klein, L., and Jovanovic, K. (2011). Regulatory T cell lineage commitment in the thymus. Semin Immunol 23:401-409.
PMID: 21733719,

Klein, L., Hinterberger, M., von Rohrscheidt, J., and Aichinger, M. (2011). Autonomous versus dendritic cell-dependent contributions of medullary thymic epithelial cells to central tolerance. Trends Immunol 32:188-193.
PMID: 21493141,

Hinterberger, M., Wirnsberger, G., and Klein, L. (2011). B7/CD28 in central tolerance: costimulation promotes maturation of regulatory T cell precursors and prevents their clonal deletion. Front Immunol 2:30.
PMID: 22566820,

Bertossi, A., Aichinger, M., Sansonetti, P., Lech, M., Neff, F., Pal, M., Wunderlich, F.T., Anders, H.J., Klein, L., and Schmidt-Supprian, M. (2011). Loss of Roquin induces early death and immune deregulation but not autoimmunity. J Exp Med 208:1749-1756.
PMID: 21844204,


Klein, L., Munz, C., and Lunemann, J.D. (2010). Autophagy-mediated antigen processing in CD4(+) T cell tolerance and immunity. FEBS Lett 584:1405-1410.
PMID: 20074571,

Hinterberger, M., Aichinger, M., Prazeres da Costa, O., Voehringer, D., Hoffmann, R., and Klein, L. (2010). Autonomous role of medullary thymic epithelial cells in central CD4(+) T cell tolerance. Nat Immunol 11:512-519.
PMID: 20431619,


Wirnsberger, G., Mair, F., and Klein, L. (2009). Regulatory T cell differentiation of thymocytes does not require a dedicated antigen-presenting cell but is under T cell-intrinsic developmental control. Proc Natl Acad Sci U S A 106:10278-10283.
PMID: 19515822,

Nedjic, J., Aichinger, M., Mizushima, N., and Klein, L. (2009). Macroautophagy, endogenous MHC II loading and T cell selection: the benefits of breaking the rules. Curr Opin Immunol 21:92-97.
PMID: 19246181,

Klein, L., Hinterberger, M., Wirnsberger, G., and Kyewski, B. (2009). Antigen presentation in the thymus for positive selection and central tolerance induction. Nat Rev Immunol 9:833-844.
PMID: 19935803,

Klein, L. (2009). Dead man walking: how thymocytes scan the medulla. Nat Immunol 10:809-811.
PMID: 19621041,


Nedjic, J., Aichinger, M., and Klein, L. (2008a). Autophagy and T cell education in the thymus: eat yourself to know yourself. Cell Cycle 7:3625-3628.
PMID: 19029805,

Nedjic, J., Aichinger, M., and Klein, L. (2008b). A novel role for autophagy in T cell education. Autophagy 4:1090-1092.
PMID: 18927488,

Nedjic, J., Aichinger, M., Emmerich, J., Mizushima, N., and Klein, L. (2008c). Autophagy in thymic epithelium shapes the T-cell repertoire and is essential for tolerance. Nature 455:396-400.
PMID: 18701890,


Aschenbrenner, K., D'Cruz, L.M., Vollmann, E.H., Hinterberger, M., Emmerich, J., Swee, L.K., Rolink, A., and Klein, L. (2007). Selection of Foxp3+ regulatory T cells specific for self antigen expressed and presented by Aire+ medullary thymic epithelial cells. Nat Immunol 8:351-358.
PMID: 17322887,


Kyewski, B., and Klein, L. (2006). A central role for central tolerance. Annu Rev Immunol 24:571-606.
PMID: 16551260,


Klein, L., Emmerich, J., d'Cruz, L., Aschenbrenner, K., and Khazaie, K. (2005). Selection and behavior of CD4+ CD25+ T cells in vivo: lessons from T cell receptor transgenic models. Curr Top Microbiol Immunol 293:73-87.
PMID: 15981476,

D'Cruz, L.M., and Klein, L. (2005). Development and function of agonist-induced CD25+Foxp3+ regulatory T cells in the absence of interleukin 2 signaling. Nat Immunol 6:1152-1159.
PMID: 16227983,

Bos, R., van Duikeren, S., van Hall, T., Kaaijk, P., Taubert, R., Kyewski, B., Klein, L., Melief, C.J., and Offringa, R. (2005). Expression of a natural tumor antigen by thymic epithelial cells impairs the tumor-protective CD4+ T-cell repertoire. Cancer Res 65:6443-6449.
PMID: 16024649,


Havari, E., Lennon-Dumenil, A.M., Klein, L., Neely, D., Taylor, J.A., McInerney, M.F., Wucherpfennig, K.W., and Lipes, M.A. (2004). Expression of the B7.1 costimulatory molecule on pancreatic beta cells abrogates the requirement for CD4 T cells in the development of type 1 diabetes. J Immunol 173:787-796.
PMID: 15240665,


von Boehmer, H., Aifantis, I., Gounari, F., Azogui, O., Haughn, L., Apostolou, I., Jaeckel, E., Grassi, F., and Klein, L. (2003). Thymic selection revisited: how essential is it? Immunol Rev 191:62-78.
PMID: 12614352,

Klein, L., Trautman, L., Psarras, S., Schnell, S., Siermann, A., Liblau, R., von Boehmer, H., and Khazaie, K. (2003a). Visualizing the course of antigen-specific CD8 and CD4 T cell responses to a growing tumor. Eur J Immunol 33:806-814.
PMID: 12616501,

Klein, L., Khazaie, K., and von Boehmer, H. (2003b). In vivo dynamics of antigen-specific regulatory T cells not predicted from behavior in vitro. Proc Natl Acad Sci U S A 100:8886-8891.
PMID: 12857946,

Jaeckel, E., Klein, L., Martin-Orozco, N., and von Boehmer, H. (2003). Normal incidence of diabetes in NOD mice tolerant to glutamic acid decarboxylase. J Exp Med 197:1635-1644.
PMID: 12796471,


Kyewski, B., Derbinski, J., Gotter, J., and Klein, L. (2002). Promiscuous gene expression and central T-cell tolerance: more than meets the eye. Trends Immunol 23:364-371.
PMID: 12103357,

Jansson, M., Panoutsakopoulou, V., Baker, J., Klein, L., and Cantor, H. (2002). Cutting edge: Attenuated experimental autoimmune encephalomyelitis in eta-1/osteopontin-deficient mice. J Immunol 168:2096-2099.
PMID: 11859094,

Gounari, F., Signoretti, S., Bronson, R., Klein, L., Sellers, W.R., Kum, J., Siermann, A., Taketo, M.M., von Boehmer, H., and Khazaie, K. (2002). Stabilization of beta-catenin induces lesions reminiscent of prostatic intraepithelial neoplasia, but terminal squamous transdifferentiation of other secretory epithelia. Oncogene 21:4099-4107.
PMID: 12037666,

Apostolou, I., Sarukhan, A., Klein, L., and von Boehmer, H. (2002). Origin of regulatory T cells with known specificity for antigen. Nat Immunol 3:756-763.
PMID: 12089509,

Anderson, M.S., Venanzi, E.S., Klein, L., Chen, Z., Berzins, S.P., Turley, S.J., von Boehmer, H., Bronson, R., Dierich, A., Benoist, C., and Mathis, D. (2002). Projection of an immunological self shadow within the thymus by the aire protein. Science 298:1395-1401.
PMID: 12376594,


Klein, L., Roettinger, B., and Kyewski, B. (2001). Sampling of complementing self-antigen pools by thymic stromal cells maximizes the scope of central T cell tolerance. Eur J Immunol 31:2476-2486.
PMID: 11500832,

Derbinski, J., Schulte, A., Kyewski, B., and Klein, L. (2001). Promiscuous gene expression in medullary thymic epithelial cells mirrors the peripheral self. Nat Immunol 2:1032-1039.
PMID: 11600886,


Kyewski, B., Rottinger, B., and Klein, L. (2000). Making central T-cell tolerance efficient: thymic stromal cells sample distinct self-antigen pools. Curr Top Microbiol Immunol 251:139-145.
PMID: 11036769,

Klein, L., and Kyewski, B. (2000a). "Promiscuous" expression of tissue antigens in the thymus: a key to T-cell tolerance and autoimmunity? J Mol Med (Berl) 78:483-494.
PMID: 11140374,

Klein, L., and Kyewski, B. (2000b). Self-antigen presentation by thymic stromal cells: a subtle division of labor. Curr Opin Immunol 12:179-186.
PMID: 10712940,

Klein, L., Klugmann, M., Nave, K.A., Tuohy, V.K., and Kyewski, B. (2000). Shaping of the autoreactive T-cell repertoire by a splice variant of self protein expressed in thymic epithelial cells. Nat Med 6:56-61.
PMID: 10613824,


Klein, L., Klein, T., Ruther, U., and Kyewski, B. (1998). CD4 T cell tolerance to human C-reactive protein, an inducible serum protein, is mediated by medullary thymic epithelium. J Exp Med 188:5-16.
PMID: 9653079,

Tourne, S., Miyazaki, T., Oxenius, A., Klein, L., Fehr, T., Kyewski, B., Benoist, C., and Mathis, D. (1997). Selection of a broad repertoire of CD4+ T cells in H-2Ma0/0 mice. Immunity 7:187-195.
PMID: 9285404,

da Costa e Silva, O., Klein, L., Schmelzer, E., Trezzini, G.F., and Hahlbrock, K. (1993). BPF-1, a pathogen-induced DNA-binding protein involved in the plant defense response. Plant J 4:125-135.
PMID: 8220470,